A culture-based method for determining the production of secreted protease inhibitors
نویسندگان
چکیده
منابع مشابه
A culture-based method for determining the production of secreted protease inhibitors.
We have developed a culture-based method for determining the production of secreted protease inhibitors. The assay utilizes standard proteolysis detection plates to support microbial growth followed by infiltrating the plate with a protease and subsequently detecting the remaining protein by trichloroacetic acid (TCA) precipitation, or by bromocreosol green (BCG) or Ponseau S (PS) staining. The...
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ژورنال
عنوان ژورنال: Journal of Microbiological Methods
سال: 2014
ISSN: 0167-7012
DOI: 10.1016/j.mimet.2014.02.019